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NOTEBOOK - SEPT
- Run gel of product from enzyme restriction cut (HsSRI-HsHtrI-Ectar gene) and the size of the bandings are correct, followed by gel purification.
- Ligation is done and the product is being transformed to Top10 competent cells, followed by spread plate.
- Pick clones from the plate containing cells that contain NpSRII-NpHtrII-Ectsr gene.
- Bacterial cultures of picked clones that contain NpSRII-NpHtrII-Ectsr gene are being sent for sequencing.
- Testing cells that contain NpSRII-NpHtrII-Ectsr gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison.
- Pick clones from the plate containing cells that contain NpSRII-NpHtrII-Ectar gene.
- Bacterial cultures of picked clones that contain NpSRII-NpHtrII-Ectar gene are being sent for sequencing.
- Testing cells that contain NpSRII-NpHtrII-Ectar gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison.
- Pick clones from the plate containing cells that contain HsSRI-HsHtrI-Ectar gene.
- Bacterial cultures of picked clones that contain HsSRI-HsHtrI-Ectar gene are being sent for sequencing.
- Testing cells that contain HsSRI-HsHtrI-Ectar gene under monochromic light using 0.5% soft agar plate while another identical plate is being place in the dark for comparison.
- Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectsr-standard backbone gene are correct. A new biobrick is successfully made.
- Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectar-standard backbone gene are correct. A new biobrick is successfully made.
- Sequencing results shown that the sequence of plasmids containing HsSRI-HsHtrI-Ectar-standard backbone gene are correct. A new biobrick is successfully made.
- Characterization of promoter Bba_J23100 using DH5α.
- Characterization of promoter Bba_J23100 using TOP10.
- Characterization of promoter Bba_J23100 using BL21.
- Repeat characterization of promoter Bba_J23100 using DH5α.
- Repeat characterization of promoter Bba_J23100 using TOP10.
- Repeat characterization of promoter Bba_J23100 using BL21.
- Second repeat characterization of promoter Bba_J23100 using DH5α. After three times of characterization, the result shown that DM5αis not optimal for this promoter.
- Second repeat characterization of promoter Bba_J23100 using TOP10. After three times of characterization, the result shown that Top 10 can effectively expresses the protein.
- Second repeat characterization of promoter Bba_J23100 using BL21. After three times of characterization, the result shown that BL21 can effectively expresses the protein.
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